D-Lactate dehydrogenase(LDH)

Rhif Cas: 9001-60-9

EC Rhif: 1.1.1.27

Pecyn: 10ku, 100ku, 500ku, 1000ku

Anfon Ymholiad

Manylion Cynnyrch

Disgrifiad

Defnyddir yr ensym ar gyfer tynnu pyruvate mewn penderfyniadau sy'n gweithio gyda NADH (triglyseridau, lipas, aldolase, aspartate aminotransferases, glwtamad dehydrogenase).

Defnydd

Gall phosphatase 1.Alkaline ynghyd â phroteinau (gwrthgyrff, streptavidin ac ati,) nodi moleciwlau targed yn benodol, a gellir eu defnyddio mewn canfod ELISA, WB a histocemegol;
Gellir defnyddio phosphatase 2.Alkaline i dephosphorize y 5 '-terminal o DNA neu RNA i atal hunan-gysylltu;
3.Gellir labelu'r DNA neu'r RNA dephosphorylated uchod gan ffosffadau radio-label (trwy T4 poly-nucleotide kinase)

Strwythur Cemegol

Mecanwaith ymateb

D-Lactate + NAD⁺→ Pyruvate +NADH + H⁺

Manyleb

Eitemau Prawf	Manylebau
Disgrifiad	Powdr amorffaidd gwyn, lyophilized
Gweithgaredd	≥200U/mg
Purdeb (SDS-PAGE)	≥90%
Hydoddedd (powdr 10mg / ml)	Clir
NADH/NADPH ocsidas	≤0.01%
Malate dehydrogenase	≤0.005%
Glutamad dehydrogenase	≤0.003%
Pyruvate kinase	≤0.03%
Glwtamad Dehydrogenase	≤0.003%
Aspartate aminotransferase	≤0.001%
Alamine aminotransferase	≤0.001%